7EDB
EcoT38I restriction endonuclease complexed with DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 173 |
Detector technology | CCD |
Collection date | 2011-02-08 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9000 |
Spacegroup name | P 64 2 2 |
Unit cell lengths | 156.291, 156.291, 173.049 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.410 - 2.390 |
R-factor | 0.1636 |
Rwork | 0.162 |
R-free | 0.19690 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7e8r |
RMSD bond length | 0.007 |
RMSD bond angle | 0.875 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.410 | 2.540 |
High resolution limit [Å] | 2.390 | 2.390 |
Rmerge | 0.082 | 0.571 |
Rmeas | 0.085 | 0.595 |
Number of reflections | 49667 | 7888 |
<I/σ(I)> | 26.24 | 6.07 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 12.15 | 12.15 |
CC(1/2) | 0.999 | 0.973 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 277 | 28% PEG400 0.2 M Calcium chloride 0.1 M Potassium chloride 0.05 M Tris-HCl |