7ECS

Crystal Structure of Aspergillus terreus Glutamate Dehydrogenase (AtGDH) Complexed With Malonate and NADPH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2016-07-09
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	I 2 2 2
Unit cell lengths	120.150, 153.740, 259.880
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.380 - 1.740
R-factor	0.14043
Rwork	0.139
R-free	0.16299
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5xvx
RMSD bond length	0.025
RMSD bond angle	2.281
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.390	1.840
High resolution limit [Å]	1.740	1.740
Rmerge	0.100	0.830
Rmeas	0.110	0.900
Number of reflections	244015	37079
<I/σ(I)>	16.72	2.39
Completeness [%]	99.9	99.1
Redundancy	7.2	6.53
CC(1/2)	0.990	0.760

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	295	2.4 M Na malonate pH 7.0