7ECQ

Crystal structure of FAM3A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	93
Detector technology	CCD
Collection date	2014-06-15
Detector	ADSC QUANTUM 315
Wavelength(s)	0.968
Spacegroup name	P 21 21 21
Unit cell lengths	40.186, 60.782, 78.907
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.453 - 1.381
R-factor	0.1636
Rwork	0.163
R-free	0.17130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2yoq
Data reduction software	Adxv
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.430
High resolution limit [Å]	1.380	1.380
Rmerge	0.055	0.394
Number of reflections	39780	3768
<I/σ(I)>	44.35
Completeness [%]	95.4
Redundancy	14.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		289.15	0.2 M Ammonium Sulfate, 0.1 M MES pH6.5, 30% w/v Polyethylene Glycol Monomethyl Ether 5000