7ECP

RNA duplex containing C-Ag-U

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-22
Detector	DECTRIS EIGER X 4M
Wavelength(s)	1.1
Spacegroup name	I 4
Unit cell lengths	88.968, 88.968, 33.326
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.450 - 2.910
R-factor	0.1608
Rwork	0.154
R-free	0.22610
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	31.450	31.450	2.990
High resolution limit [Å]	2.910	13.030	2.910
Rmerge	0.094	0.039	0.204
Rmeas	0.110	0.047	0.236
Total number of observations	10945
Number of reflections	2968	37	223
<I/σ(I)>	9.9	20.44	4.47
Completeness [%]	99.3	92.5	98.2
Redundancy	3.688	2.892	3.646
CC(1/2)	0.995	0.999	0.989

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	MOPS, MPD, spermine, lithium nitrate