7ECO

RNA duplex containing U-Ag-U base pairs

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-09
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.98
Spacegroup name	C 1 2 1
Unit cell lengths	95.771, 26.875, 78.944
Unit cell angles	90.00, 101.69, 90.00

Refinement procedure

Resolution	36.910 - 1.810
R-factor	0.1882
Rwork	0.185
R-free	0.21690
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	36.910	36.910	1.860
High resolution limit [Å]	1.810	8.090	1.810
Rmerge	0.059	0.040	0.388
Rmeas	0.064	0.044	0.425
Total number of observations	118980
Number of reflections	18483	236	1350
<I/σ(I)>	17.77	32.81	5.16
Completeness [%]	99.7	97.5	99.2
Redundancy	6.437	5.75	6.054
CC(1/2)	0.999	0.999	0.971

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	MOPS, MPD, spermine, lithium nitrate