7ECN

RNA duplex containing C-Ag-A base pairs

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-21
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.98
Spacegroup name	P 1 21 1
Unit cell lengths	35.784, 41.482, 45.934
Unit cell angles	90.00, 102.24, 90.00

Refinement procedure

Resolution	34.970 - 1.600
R-factor	0.173
Rwork	0.170
R-free	0.20340
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	34.970	34.970	1.640
High resolution limit [Å]	1.600	7.170	1.600
Rmerge	0.040	0.019	0.291
Rmeas	0.048	0.023	0.350
Total number of observations	56696
Number of reflections	17296	206	1261
<I/σ(I)>	16.46	40.23	3.86
Completeness [%]	99.1	97.2	98.1
Redundancy	3.278	3.107	3.206
CC(1/2)	0.999	1.000	0.965

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	MOPS, MPD, spermine, potassium nitrate