7EC0
Crystal structure of juvenile hormone acid methyltransferase JHAMT in complex with S-Adenosyl homocysteine and methyl farnesoate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-01-09 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 82.459, 101.203, 103.363 |
Unit cell angles | 90.00, 93.50, 90.00 |
Refinement procedure
Resolution | 72.260 - 2.494 |
Rwork | 0.245 |
R-free | 0.29260 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7ebx |
RMSD bond length | 0.003 |
RMSD bond angle | 1.200 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 72.260 | 2.630 |
High resolution limit [Å] | 2.490 | 2.490 |
Rmerge | 0.056 | 0.734 |
Rmeas | 0.062 | 0.792 |
Number of reflections | 58792 | 8516 |
<I/σ(I)> | 18.2 | 0.6 |
Completeness [%] | 99.3 | 99.1 |
Redundancy | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 289 | 0.15M DL-Malic acid pH 7.0, 20% PEG 3350 |