7EBY

Crystal structure of D-Succinylase (DSA) from Cupriavidus sp. P4-10-C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-11-10
Detector	RIGAKU SATURN A200
Wavelength(s)	1.0
Spacegroup name	P 1
Unit cell lengths	83.586, 92.117, 162.111
Unit cell angles	94.79, 96.31, 104.90

Refinement procedure

Resolution	47.630 - 2.000
R-factor	0.2064
Rwork	0.204
R-free	0.25160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4HST.pdb
RMSD bond length	0.007
RMSD bond angle	0.935
Data reduction software	DENZO
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.030
High resolution limit [Å]	2.000	2.000
Rmeas	0.120	0.602
Rpim	0.076	0.379
Number of reflections	303340	15023
<I/σ(I)>	7.5	2.4
Completeness [%]	97.8	96.8
Redundancy	2.5	2.4
CC(1/2)	0.991	0.663

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	24% PEG 1500, 20% Glycerol