7EBX
Crystal structure of juvenile hormone acid methyltransferase JHAMT in complex with S-adenosyl-L-homocysteine.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-15 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 102.155, 102.155, 102.144 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.690 - 2.890 |
| Rwork | 0.251 |
| R-free | 0.29160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7ebs |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.224 |
| Data reduction software | Aimless |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.694 | 2.950 |
| High resolution limit [Å] | 2.890 | 2.890 |
| Rmerge | 0.082 | 0.908 |
| Number of reflections | 12618 | 603 |
| <I/σ(I)> | 42.867 | 1.795 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 20.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 289 | 0.1M Bis-Tris pH 5.5, 1M ammonium sulfate, 1% PEG 3350 |
