7EBS
Crystal structure of juvenile hormone acid methyltransferase JHAMT from silkworm
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-07-09 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 32 1 2 |
Unit cell lengths | 99.584, 99.584, 70.607 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 86.240 - 2.950 |
R-factor | 0.238 |
Rwork | 0.235 |
R-free | 0.28120 |
Structure solution method | SAD |
RMSD bond length | 0.004 |
RMSD bond angle | 1.278 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 86.240 | 3.060 |
High resolution limit [Å] | 2.950 | 2.950 |
Rmerge | 0.048 | 0.660 |
Rmeas | 0.052 | 0.711 |
Number of reflections | 8562 | 506 |
<I/σ(I)> | 30.66 | 2.3 |
Completeness [%] | 99.7 | 99.9 |
Redundancy | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 289 | 0.1M Bis-Tris pH 5.5, 1M ammonium sulfate, 1% PEG 3350 |