7EBK

Mouse Trim66 PHD-Bromo dual domain complexed with the H3(1-24)K9me3K18ac peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	77
Detector technology	PIXEL
Collection date	2017-03-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97780
Spacegroup name	P 65 2 2
Unit cell lengths	64.646, 64.646, 251.744
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.830 - 1.740
R-factor	0.2142
Rwork	0.213
R-free	0.22840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3o33
RMSD bond length	0.007
RMSD bond angle	0.885
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.780
High resolution limit [Å]	1.740	1.750
Number of reflections	32908	1568
<I/σ(I)>	61.3
Completeness [%]	98.9
Redundancy	25.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	15% v/v TacsimateTM pH 7.0, 0.1 M HEPES pH 7.0, 2% w/v Polyethylene glycol 3350