7EBJ

Apo structure of the mouse Trim66 PHD-Bromo dual domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	77
Detector technology	PIXEL
Collection date	2017-03-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97780
Spacegroup name	P 41 21 2
Unit cell lengths	80.073, 80.073, 80.046
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.220 - 1.800
R-factor	0.1847
Rwork	0.183
R-free	0.21200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3o33
RMSD bond length	0.007
RMSD bond angle	0.855
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.830
High resolution limit [Å]	1.800	1.800
Number of reflections	24555	1208
<I/σ(I)>	24.8
Completeness [%]	99.5
Redundancy	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	0.1 M MES monohydrate pH 6.5, 12% w/v Polyethylene glycol 20000