7EB9
The structure of the A20-binding inhibitor of NF-kB 1 in complex with tetra-ubiquitin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13C1 |
Synchrotron site | NSRRC |
Beamline | BL13C1 |
Temperature [K] | 113 |
Detector technology | CCD |
Collection date | 2014-03-04 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9766 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 45.381, 53.777, 240.115 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.000 - 3.200 |
R-factor | 0.2211 |
Rwork | 0.216 |
R-free | 0.26370 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7eao |
RMSD bond length | 0.002 |
RMSD bond angle | 0.482 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.15.2_3472: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.000 | 3.300 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.089 | 0.541 |
Number of reflections | 10149 | 790 |
<I/σ(I)> | 16.4 | 3.47 |
Completeness [%] | 96.3 | |
Redundancy | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | 0.1 M Sodium malonate pH 6.0, 10% PEG 3350 |