7EB6

Crystal structure of GTP-binding protein-like domain of AGAP1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2020-03-08
Detector	MAR CCD 165 mm
Wavelength(s)	0.99
Spacegroup name	I 4
Unit cell lengths	100.390, 100.390, 48.080
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.493 - 3.014
R-factor	0.2359
Rwork	0.233
R-free	0.26490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2iwr
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.493	3.122
High resolution limit [Å]	3.014	3.014
Rmerge	0.130
Number of reflections	9136	458
<I/σ(I)>	8.7
Completeness [%]	99.6
Redundancy	5.9
CC(1/2)	0.949	0.700

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		278	PEG 8000