7EAO
The structure of the A20-binding inhibitor of NF-kB 1 in complex with tri-ubiquitin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 113 |
| Detector technology | CCD |
| Collection date | 2013-10-13 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.00 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 95.638, 81.851, 61.056 |
| Unit cell angles | 90.00, 109.39, 90.00 |
Refinement procedure
| Resolution | 29.196 - 2.900 |
| R-factor | 0.2294 |
| Rwork | 0.223 |
| R-free | 0.28270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7eal |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.665 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.072 | 0.466 |
| Number of reflections | 9441 | 576 |
| <I/σ(I)> | 16.72 | 2.17 |
| Completeness [%] | 91.5 | |
| Redundancy | 16.72 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.2 M Potassium citrate tribasic, 20% PEG 3350 |
