7EAM

immune complex of SARS-CoV-2 RBD and cross-neutralizing antibody 7D6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-01-02
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 1
Unit cell lengths	37.440, 87.970, 102.720
Unit cell angles	89.96, 87.05, 89.98

Refinement procedure

Resolution	22.320 - 1.400
R-factor	0.174
Rwork	0.174
R-free	0.18070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6m0j 6rco
Data reduction software	Aimless
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.320	1.440
High resolution limit [Å]	1.400	1.400
Rmerge	0.061	1.247
Rpim	0.039	0.936
Number of reflections	476102	17141
<I/σ(I)>	8.8
Completeness [%]	94.7	89.5
Redundancy	3.4	2.7
CC(1/2)	0.996	0.331

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		293.15	PEG D5, 0.2M Potassium dihydrogen phosphate, 20% PEG3350