7EAL
The structure of the A20-Binding Inhibitor of NF-kB 1 in complex with di-ubiquitin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2013-07-30 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 80.753, 59.990, 84.058 |
| Unit cell angles | 90.00, 109.71, 90.00 |
Refinement procedure
| Resolution | 23.563 - 2.500 |
| R-factor | 0.1859 |
| Rwork | 0.184 |
| R-free | 0.22430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5h07 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.997 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.570 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.072 | 0.510 |
| Number of reflections | 26579 | 2342 |
| <I/σ(I)> | 16.05 | 3.04 |
| Completeness [%] | 97.9 | 87.13 |
| Redundancy | 3.5 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 255 | 0.1M Citrate pH 5.5, 10% Iso-propanol, 20% PEG 400 |
