7EAF

Crystal structure of SAM-I riboswitch with the Actinomyces-1 k-turn

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-01-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9785
Spacegroup name	P 43 21 2
Unit cell lengths	61.460, 61.460, 157.910
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.220 - 2.850
R-factor	0.2639
Rwork	0.262
R-free	0.29760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5fk3
RMSD bond length	0.004
RMSD bond angle	0.965
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.950
High resolution limit [Å]	2.800	2.800
Rmeas	0.154
Rpim	0.056	0.656
Number of reflections	7963	1123
<I/σ(I)>	8.9	1.4
Completeness [%]	99.6	99.6
Redundancy	7.2	7.7
CC(1/2)	0.990	0.771

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	290	40 MM NA-CACODYLATE (PH 7.0), 80 MM KCL, 40 MM BACL2, 12 MM SPERMINE-HCL, 12% (V/V) MPD.