7EA4
Crystal Structure of L182E D-Succinylase (DSA) from Cupriavidus sp. P4-10-C
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2014-06-14 |
Detector | RIGAKU RAXIS V |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 62.057, 129.806, 200.680 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.930 - 1.950 |
R-factor | 0.1666 |
Rwork | 0.164 |
R-free | 0.20800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4hst |
RMSD bond length | 0.007 |
RMSD bond angle | 0.881 |
Data reduction software | DENZO |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.980 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.112 | |
Rmeas | 0.112 | 0.619 |
Rpim | 0.051 | 0.284 |
Number of reflections | 118124 | 5812 |
<I/σ(I)> | 11 | |
Completeness [%] | 99.6 | 99.9 |
Redundancy | 4.7 | 4.5 |
CC(1/2) | 0.993 | 0.821 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 298 | 11% PEG 8000, 0.1M Sodium cacodylate, 0.2M MaCl2 |