7EA1

Crystal Structure of Spindlin1 bound to SPINDOC Docpep2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2018-02-03
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97853
Spacegroup name	P 21 21 21
Unit cell lengths	83.795, 136.904, 42.240
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.363 - 2.700
R-factor	0.2553
Rwork	0.254
R-free	0.28590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ns2
Data reduction software	DENZO
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3247)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.750
High resolution limit [Å]	2.689	7.320	2.700
Rmerge	0.103	0.084	0.422
Rmeas	0.113	0.093	0.465
Rpim	0.045	0.038	0.192
Number of reflections	14134	799	671
<I/σ(I)>	5.1
Completeness [%]	99.8	99	99.9
Redundancy	6.4	5.6	5.6
CC(1/2)		0.993	0.914

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	291	0.1M Sodium chloride, 0.1M BIS-TRIS pH 6.5, 1.5M Ammonium sulfate