7E9X

Trehalase of Arabidopsis thaliana acid mutant -D380A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-19
Detector	DECTRIS PILATUS3 S 2M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	54.657, 129.647, 151.141
Unit cell angles	90.00, 91.03, 90.00

Refinement procedure

Resolution	41.550 - 1.880
R-factor	0.1938
Rwork	0.193
R-free	0.24500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7e9u
RMSD bond length	0.007
RMSD bond angle	0.832
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.550	1.950
High resolution limit [Å]	1.880	1.880
Number of reflections	169902	27135
<I/σ(I)>	14.34
Completeness [%]	99.8
Redundancy	6.82
CC(1/2)	0.999	0.838

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	10 mM Tre6P, 0.1 M sodium chloride and 20% PEG3400.