7E9S

Archaeal oligosaccharyltransferase AglB from Archaeoglobus fulgidus in complex with an inhibitory peptide and a dolichol-phosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-20
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.0000
Spacegroup name	P 21 21 2
Unit cell lengths	345.740, 48.690, 63.560
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.840 - 2.700
R-factor	0.2171
Rwork	0.215
R-free	0.25220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5gmy
RMSD bond length	0.003
RMSD bond angle	0.592
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.400	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.432	8.718
Rmeas	0.435	8.776
Number of reflections	30677	3054
<I/σ(I)>	13.6	1.3
Completeness [%]	100.0	99.9
Redundancy	79.5	76.9
CC(1/2)	0.998	0.756

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	6	293	50 mM NaCl, 0.1 M Na-citrate, pH 6.0, 18-22 % PEG400