7E9L

Crystal Structure of POMGNT2 in complex with UDP and mono-mannosyl peptide (379Man short peptide)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-22
Detector	DECTRIS EIGER X 4M
Wavelength(s)	1.1000
Spacegroup name	P 21 21 21
Unit cell lengths	92.394, 104.440, 126.836
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.290 - 2.100
R-factor	0.1878
Rwork	0.185
R-free	0.23380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	D_1300018733
RMSD bond length	0.008
RMSD bond angle	0.876
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.290	2.140
High resolution limit [Å]	2.100	2.100
Rmerge	0.114	1.574
Rpim	0.032	0.424
Number of reflections	72133	4284
<I/σ(I)>	16	1.9
Completeness [%]	99.8
Redundancy	13.9
CC(1/2)	0.999	0.791

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.1 M Tris-HCl (pH 8.0 or 8.5), 12-16% PEG 8000, 1% tacsimate (pH 7.0)