7E92

Crystal structure of the DNA-binding domain of the response regulator VbrR from Vibrio parahaemolyticus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2019-11-02
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	35.315, 51.758, 97.224
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.800
R-factor	0.1882
Rwork	0.186
R-free	0.23100
Structure solution method	MAD
RMSD bond length	0.005
RMSD bond angle	0.728
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AutoSol
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.830
High resolution limit [Å]	1.800	1.800
Rmerge	0.054	0.490
Number of reflections	17222	838
<I/σ(I)>	33.7	3.5
Completeness [%]	99.7	99.8
Redundancy	3.9	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	PEG 400, MES, PEG 3350
1	VAPOR DIFFUSION, SITTING DROP		291	PEG 400, MES, PEG 3350