7E8O
Crystal structure of proteinaceous RNase P(PRORP) from Planctomycetes bacterium GWF2_40_8 complexed with Escherichia coli histidine pre-tRNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2019-10-30 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 146.277, 146.277, 143.369 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.459 - 2.804 |
| R-factor | 0.2323 |
| Rwork | 0.230 |
| R-free | 0.28450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7e8k |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.604 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 6.020 | 2.800 |
| Rmerge | 0.117 | 0.035 | 0.592 |
| Rmeas | 0.121 | 0.036 | 0.632 |
| Rpim | 0.030 | 0.008 | 0.216 |
| Total number of observations | 584223 | ||
| Number of reflections | 38678 | 4128 | 3763 |
| <I/σ(I)> | 6.9 | ||
| Completeness [%] | 99.9 | 99.8 | 99.8 |
| Redundancy | 15.1 | 21.7 | 8 |
| CC(1/2) | 0.998 | 0.053 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 8% (v/v) Tacsimate pH 8.0, 20% (w/v) PEG 3350 |
