7E8K

Crystal structure of Proteinaceous RNase P (PRORP) from Planctomycetes bacterium GWF2_40_8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	80
Detector technology	CCD
Collection date	2018-11-26
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.9793
Spacegroup name	P 31
Unit cell lengths	99.932, 99.932, 176.178
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.363 - 2.250
R-factor	0.1912
Rwork	0.190
R-free	0.21480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7e8j
RMSD bond length	0.007
RMSD bond angle	1.131
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.330
High resolution limit [Å]	2.249	4.840	2.250
Rmerge	0.093	0.051	0.361
Rmeas	0.098	0.054	0.410
Rpim	0.033	0.017	0.188
Number of reflections	92631	9337	8688
<I/σ(I)>	7.2
Completeness [%]	99.0	99.8	92.9
Redundancy	7.1	9.7	3.8
CC(1/2)		0.999	0.651

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	2000mM Ammonium sulfate, 100mM Sodium citrate/Citric acid pH 5.5