7E7W

Crystal structure of RSL mutant in complex with sugar Ligand

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-09
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97915
Spacegroup name	P 1
Unit cell lengths	46.567, 46.554, 103.254
Unit cell angles	90.04, 89.92, 119.98

Refinement procedure

Resolution	26.200 - 4.100
R-factor	0.2063
Rwork	0.200
R-free	0.34670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4csd
Data reduction software	HKL-2000
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	4.250
High resolution limit [Å]	4.100	8.770	4.100
Rmerge	0.120	0.038	0.400
Rmeas	0.143	0.045	0.480
Rpim	0.078	0.025	0.263
Total number of observations	19061
Number of reflections	6095	618	629
<I/σ(I)>	4.7
Completeness [%]	99.2	99.4	98.9
Redundancy	3.1	3.1	3
CC(1/2)		0.990	0.869

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	SMALL TUBES	7.5	277	20 mM Tris-HCl, 100 mM of NaCl pH 7.5, the crystal was obtained in Micro centrifuge tube sequentially put with RSL solution, pure buffer, and the ligand solution