7E7V
Crystal structure of RSL mutant in complex with sugar Ligand
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-11-04 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979183 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 43.494, 161.519, 54.137 |
Unit cell angles | 90.00, 91.53, 90.00 |
Refinement procedure
Resolution | 80.760 - 1.610 |
R-factor | 0.2194 |
Rwork | 0.218 |
R-free | 0.24850 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4csd |
RMSD bond length | 0.014 |
RMSD bond angle | 1.960 |
Data reduction software | autoPROC |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 80.760 | 80.760 | 1.690 |
High resolution limit [Å] | 1.610 | 5.080 | 1.610 |
Rmerge | 0.079 | 0.057 | 0.590 |
Rmeas | 0.086 | 0.062 | 0.662 |
Rpim | 0.034 | 0.024 | 0.296 |
Total number of observations | 597512 | 21043 | 64788 |
Number of reflections | 94580 | 3116 | 13552 |
<I/σ(I)> | 15 | 32 | 2.7 |
Completeness [%] | 98.0 | 99.8 | 96.7 |
Redundancy | 6.3 | 6.8 | 4.8 |
CC(1/2) | 0.998 | 0.998 | 0.832 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | SMALL TUBES | 7.5 | 277 | 20 mM Tris-HCl, 100 mM of NaCl pH 7.5, Micro centrifuge tube sequentially put with RSL solution, pure buffer, ligand solution |