7E7A

Crystal structure of apo ENL YEATS domain T3 mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2018-06-03
Detector	MAR CCD 130 mm
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	83.316, 88.258, 167.008
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.040 - 2.640
R-factor	0.2138
Rwork	0.213
R-free	0.23040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5j9s
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.700
High resolution limit [Å]	2.640	7.190	2.650
Rmerge	0.091	0.058	0.614
Rmeas	0.107	0.068	0.723
Rpim	0.055	0.035	0.374
Number of reflections	35739	1882	1771
<I/σ(I)>	12.1
Completeness [%]	96.9	93.1	97.7
Redundancy	3.6	3.6	3.6
CC(1/2)		0.993	0.711

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	291	2M sodium formate, 0.1M sodium acetate trihydrate pH 4.6