7E78
the structure of cytosolic TaPGI with substrate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-01-03 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.979 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 243.616, 74.449, 125.585 |
Unit cell angles | 90.00, 94.12, 90.00 |
Refinement procedure
Resolution | 35.680 - 2.210 |
R-factor | 0.2335 |
Rwork | 0.233 |
R-free | 0.25790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ujh |
RMSD bond length | 0.008 |
RMSD bond angle | 0.950 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.000 | 2.080 |
High resolution limit [Å] | 2.040 | 2.040 |
Rmerge | 0.088 | |
Number of reflections | 112442 | 14 |
<I/σ(I)> | 7.2 | |
Completeness [%] | 99.3 | |
Redundancy | 3.9 | |
CC(1/2) | 0.230 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | MES, PEG MME2000 |