7E72

Crystal structure of Tie2-agonistic antibody in complex with human Tie2 Fn2-3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-25
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97940
Spacegroup name	P 1
Unit cell lengths	45.080, 77.340, 121.849
Unit cell angles	103.82, 96.89, 90.59

Refinement procedure

Resolution	44.720 - 2.094
R-factor	0.1838
Rwork	0.183
R-free	0.23310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5x8m 5mya
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.720	2.169
High resolution limit [Å]	2.094	2.094
Rmerge	0.099	0.509
Number of reflections	84953	8247
<I/σ(I)>	7.126	1.217
Completeness [%]	91.0	88.61
Redundancy	3.6	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	292.15	100 mM Bis-Tris propane pH 8.5, 110 mM potassium dihydrogen phosphate, 15% (w/v) polyethylene glycol 3350