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7E6Y

Time-resolved serial femtosecond crystallography reveals early structural changes in channelrhodopsin: 1 microsecond structure

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeFREE ELECTRON LASER
Source detailsSACLA BEAMLINE BL3
Synchrotron siteSACLA
BeamlineBL3
Temperature [K]293
Detector technologyCCD
Collection date2016-10-12
DetectorMPCCD
Wavelength(s)1.77
Spacegroup nameC 2 2 21
Unit cell lengths61.800, 142.200, 94.700
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution14.960 - 2.500
R-factor0.4485
Rwork0.445
R-free0.51480
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)3ug9
RMSD bond length0.012
RMSD bond angle1.595
Data reduction softwareCrystFEL (0.6.3)
Data scaling softwareCrystFEL (0.6.3)
Phasing softwarePHASER
Refinement softwareREFMAC (5.8.0267)
Data quality characteristics
 OverallInner shellOuter shell
Low resolution limit [Å]39.41039.5262.441
High resolution limit [Å]2.4006.5062.400
Number of reflections16730916808
<I/σ(I)>6.1513.210.86
Completeness [%]100.0100100
Redundancy131.1344.437.8
CC(1/2)0.9870.9790.439
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1LIPIDIC CUBIC PHASE6.9293100 mM MES, pH 6.9, 100 mM Na formate, and 30% PEG500DM

219869

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