7E62

Mouse TAB2 NZF in complex with Lys6-linked diubiquitin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-31
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	45.694, 76.170, 114.813
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.460 - 1.990
R-factor	0.243
Rwork	0.242
R-free	0.26360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3a9j
RMSD bond length	0.004
RMSD bond angle	0.861
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.460	2.110
High resolution limit [Å]	1.990	1.990
Number of reflections	28130	4318
<I/σ(I)>	9.11
Completeness [%]	99.5
Redundancy	13.2
CC(1/2)	0.998	0.485

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	100 mM HEPES-NaOH (pH 7.5), 1.44 M sodium citrate