7E3T

Crystal structure of TrmL from Mycoplasma capricolum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-28
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9794
Spacegroup name	P 64 2 2
Unit cell lengths	79.824, 79.824, 117.333
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.770 - 2.100
R-factor	0.1971
Rwork	0.195
R-free	0.23950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4kdz
RMSD bond length	0.009
RMSD bond angle	1.499
Data reduction software	HKL-2000
Data scaling software	HKL-2000 (0.7.4)
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.140
High resolution limit [Å]	2.100	5.700	2.100
Rmerge	0.096	0.039	1.388
Rmeas	0.101	0.042	1.474
Rpim	0.031	0.014	0.482
Total number of observations	137705
Number of reflections	13552	792	652
<I/σ(I)>	3.9
Completeness [%]	99.9	99.4	99.8
Redundancy	10.2	9.1	8.6
CC(1/2)		0.999	0.714

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	0.1M MES monohydrate pH 6.5, 0.05M Cesium chloride, 30%v/v Jeffamine M-600