7E3J
Crystal structure of SARS-CoV-2 RBD binding to dog ACE2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-06-08 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.979 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 168.256, 168.256, 211.196 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.260 - 2.990 |
R-factor | 0.2282 |
Rwork | 0.227 |
R-free | 0.24790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6lzg |
RMSD bond length | 0.002 |
RMSD bond angle | 0.531 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.110 |
High resolution limit [Å] | 2.990 | 3.000 |
Number of reflections | 30355 | 2989 |
<I/σ(I)> | 8.8 | 2.6 |
Completeness [%] | 98.5 | |
Redundancy | 6.3 | |
CC(1/2) | 0.980 | 0.819 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291 | 2% 1,4-dioxane, 0.1 M tris pH 8.0,15% polyethylene glycol 3,350. |