7E34

Crystal structure of SUN1-Speedy A-CDK2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-01-22
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.91905
Spacegroup name	C 2 2 21
Unit cell lengths	59.340, 183.525, 103.829
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.590 - 3.190
R-factor	0.254
Rwork	0.252
R-free	0.30000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5uq2
RMSD bond length	0.008
RMSD bond angle	0.940
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.310
High resolution limit [Å]	3.190	6.890	3.200
Rmerge	0.108	0.046	0.573
Rmeas	0.120	0.051	0.657
Rpim	0.051	0.023	0.314
Number of reflections	9795	1060	928
<I/σ(I)>	5.7
Completeness [%]	99.3	99.1	94.3
Redundancy	5.2	5	4
CC(1/2)		0.997	0.808

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291	0.15M ammonium citrate tribasic, pH7.0, 12% PEG 3350