7E2U

Synechocystis GUN4 in complex with phytochrome

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	32.522, 64.601, 106.892
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.180 - 1.700
R-factor	0.1593
Rwork	0.158
R-free	0.19400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4xkc
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.760
High resolution limit [Å]	1.700	1.700
Rmerge	0.111	0.712
Rpim	0.046	0.340
Number of reflections	25494	2325
<I/σ(I)>	16.8	2.1
Completeness [%]	99.1
Redundancy	6.4
CC(1/2)	0.989	0.688

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	Sodium fluoride, PEG 3350