7E2T

Synechocystis GUN4 in complex with phycocyanobilin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-29
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	32.465, 64.695, 106.521
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.950 - 1.100
R-factor	0.1267
Rwork	0.125
R-free	0.15630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4xkc
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.140
High resolution limit [Å]	1.100	1.100
Rmerge	0.148	0.820
Rpim	0.050	0.270
Number of reflections	91915	9050
<I/σ(I)>	15.5	2.3
Completeness [%]	100.0	100
Redundancy	9.6	9.7
CC(1/2)	0.994	0.838

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	Sodium citrate pH 5.5, PEG 6000