7E2Q

Crystal structure of Mycoplasma pneumoniae Enolase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	CCD
Collection date	2018-12-19
Detector	AGILENT ATLAS CCD
Wavelength(s)	0.97853
Spacegroup name	P 1 21 1
Unit cell lengths	76.074, 106.548, 128.589
Unit cell angles	90.00, 103.15, 90.00

Refinement procedure

Resolution	19.840 - 1.800
R-factor	0.1909
Rwork	0.189
R-free	0.22260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6j36
RMSD bond length	0.006
RMSD bond angle	0.787
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	HKL-3000
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.840	1.900
High resolution limit [Å]	1.800	1.800
Number of reflections	183711	26642
<I/σ(I)>	6.4
Completeness [%]	99.6
Redundancy	6.4
CC(1/2)	0.997	0.760

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.2M Sodium citrate tribasic dihydrate, 0.1M TRIS hydrochloride pH 8.5, 30% v/v Polyethylene glycol 400