7E1G

Structure of MreB3 from Spiroplasma eriocheiris

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0000
Spacegroup name	P 1 21 1
Unit cell lengths	50.290, 56.270, 120.531
Unit cell angles	90.00, 90.56, 90.00

Refinement procedure

Resolution	50.987 - 1.750
R-factor	0.1756
Rwork	0.174
R-free	0.21350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7e1c
RMSD bond length	0.006
RMSD bond angle	0.891
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.15.2_3472: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.300	1.780
High resolution limit [Å]	1.750	1.750
Rmerge	0.101	0.570
Rpim	0.065	0.372
Number of reflections	66116	3545
<I/σ(I)>	6.8	1.8
Completeness [%]	97.2
Redundancy	3.3
CC(1/2)	0.993	0.658

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.8	293	30% (w/v) PEG 4000, 0.1 M Acetate-NaOH pH 4.6, 200 mM NH4Ac, 5 mM AMPPNP, 5mM MgCl2