7E15

Protein ternary complex working for DNA replication initiation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-29
Detector	DECTRIS EIGER X 4M
Wavelength(s)	1.0000
Spacegroup name	P 1 21 1
Unit cell lengths	69.302, 48.308, 205.759
Unit cell angles	90.00, 96.19, 90.00

Refinement procedure

Resolution	47.010 - 2.450
R-factor	0.2174
Rwork	0.216
R-free	0.24190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ghr
RMSD bond length	0.002
RMSD bond angle	0.477
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.450	2.450
Rmerge	0.078	0.398
Rmeas	0.110	0.560
Rpim	0.077	0.395
Number of reflections	93014	16322
<I/σ(I)>	6.9	2
Completeness [%]	99.5	99.7
Redundancy	3.5	3.5
CC(1/2)	0.997	0.927

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	0.15 M DL-malic acid (pH 7.0), 20 % (w/v) PEG 3350