7E0C
Structure of L-glutamate oxidase R305E mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-10-08 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.000 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 123.970, 123.970, 168.631 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 66.311 - 2.650 |
R-factor | 0.1934 |
Rwork | 0.191 |
R-free | 0.24090 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2e1m |
RMSD bond length | 0.006 |
RMSD bond angle | 0.860 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.15.2_3472: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 84.300 | 2.780 |
High resolution limit [Å] | 2.650 | 2.650 |
Rmerge | 0.115 | 0.670 |
Rpim | 0.034 | 0.191 |
Number of reflections | 22897 | 2970 |
<I/σ(I)> | 18.5 | 4.6 |
Completeness [%] | 99.9 | 100 |
Redundancy | 12.3 | 13 |
CC(1/2) | 0.999 | 0.930 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 12% (w/v) PEG 8000, 0.1M Tris-HCl pH7.0, 75mM MgCl2 |