7E0B
The crystal structure of sorting nexin 27 and PBM complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-04-23 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9791 |
Spacegroup name | H 3 |
Unit cell lengths | 88.550, 88.550, 43.270 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.820 - 1.290 |
R-factor | 0.1845 |
Rwork | 0.184 |
R-free | 0.19060 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4z8j |
RMSD bond length | 0.003 |
RMSD bond angle | 0.543 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 22.140 | 1.320 |
High resolution limit [Å] | 1.290 | 1.290 |
Rmerge | 0.055 | |
Number of reflections | 30746 | 6895 |
<I/σ(I)> | 17.3 | |
Completeness [%] | 97.8 | |
Redundancy | 8.6 | |
CC(1/2) | 0.998 | 0.519 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291 | 0.2 M sodium sulfate, 20 % w/v PEG 3350. |