7E09

Trans-3/4-proline-hydroxylase H11 with 3-hydroxyl-proline

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-10
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	P 41 2 2
Unit cell lengths	75.390, 75.390, 143.650
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	75.390 - 2.380
R-factor	0.1933
Rwork	0.192
R-free	0.22550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6LNS
RMSD bond length	0.018
RMSD bond angle	1.898
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	75.390	2.420
High resolution limit [Å]	2.380	2.380
Rmerge	0.067	0.502
Rmeas	0.070	0.521
Rpim	0.020	0.141
Number of reflections	17231	856
<I/σ(I)>	86.4	10.3
Completeness [%]	99.9	100
Redundancy	12.6	13.2
CC(1/2)		0.969

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	0.7	289.15	0.7M Sodium citrate tribasic dihydrate, 0.1M Bis-Tris propane (pH 7.0)