7E02
Room temperature structure of lysozyme delivered in beef tallow by serial millisecond crystallography
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 11C |
Synchrotron site | PAL/PLS |
Beamline | 11C |
Temperature [K] | 293.15 |
Detector technology | PIXEL |
Collection date | 2019-05-19 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9796 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 79.400, 79.400, 38.420 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 56.140 - 1.550 |
R-factor | 0.1699 |
Rwork | 0.168 |
R-free | 0.18790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7cvj |
Data reduction software | CrystFEL |
Data scaling software | CrystFEL |
Phasing software | PHENIX |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 80.000 | 1.600 |
High resolution limit [Å] | 1.550 | 1.550 |
Number of reflections | 18428 | 1809 |
<I/σ(I)> | 10.63 | 1.87 |
Completeness [%] | 100.0 | 100 |
Redundancy | 1257.8 | 667.4 |
CC(1/2) | 0.994 | 0.624 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | SMALL TUBES | 4.5 | 293.5 | sodium acetate, PEG 8000, NaCl |