7DZL

A69C-M71L mutant of Fabp protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-30
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	56.987, 66.830, 68.406
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.850 - 1.640
R-factor	0.1884
Rwork	0.187
R-free	0.21500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1lkk
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.450	1.720
High resolution limit [Å]	1.630	1.630
Rmerge	0.031	0.123
Number of reflections	32859	3185
<I/σ(I)>	38.98	10.8
Completeness [%]	99.7	98.8
Redundancy	7.5	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	277	0.1 M potassium thiocyanate, pH 7.0, 30% PEG 2000 monomethyl ether