7DYM

Pseudomonas aeruginosa TseT-TsiT complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-13
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 65
Unit cell lengths	77.500, 77.500, 185.900
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.370 - 3.100
R-factor	0.2079
Rwork	0.206
R-free	0.23720
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	XDS
Phasing software	AutoSol
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.530	3.180
High resolution limit [Å]	3.100	3.100
Rmerge	0.063	0.480
Number of reflections	11293	777
<I/σ(I)>	23.34
Completeness [%]	98.8
Redundancy	10
CC(1/2)	0.990	0.960

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	0.18 m Trimethylamine N-oxide, 0.1M Tris pH 8.5, 11% PEG2000MME