7DYE
Crystal Structure of Cyanobacterial Circadian Clock Protein KaiC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-04-09 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9 |
| Spacegroup name | H 3 |
| Unit cell lengths | 94.906, 94.906, 276.523 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.500 - 2.600 |
| R-factor | 0.2819 |
| Rwork | 0.279 |
| R-free | 0.33660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gbl |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.249 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.500 | 2.720 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.069 | 0.612 |
| Number of reflections | 28555 | 3485 |
| <I/σ(I)> | 12.5 | |
| Completeness [%] | 99.9 | |
| Redundancy | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 7 | 313 | sodium/potassium tartrate, sodium acetate, KCl |
