7DXV

Crystal structure of the mk2h_deltaY peptide homodimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2020-07-22
Detector	RAYONIX MX225-HS
Wavelength(s)	1
Spacegroup name	H 3
Unit cell lengths	73.101, 73.101, 49.177
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.551 - 2.301
R-factor	0.2061
Rwork	0.202
R-free	0.24830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7dxr
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.340
High resolution limit [Å]	2.210	2.210
Number of reflections	4833	705
<I/σ(I)>	29.72
Completeness [%]	98.0
Redundancy	10.87
CC(1/2)	0.999	0.993

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	100 mM SPG buffer, 25 % PEG 1500