7DXQ

Crystal Structure of Cyanobacterial Circadian Clock Protein KaiC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2016-05-30
Detector	RAYONIX MX-225
Wavelength(s)	0.9
Spacegroup name	P 21 21 21
Unit cell lengths	92.411, 159.874, 207.243
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.960 - 2.800
R-factor	0.2532
Rwork	0.250
R-free	0.31760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2gbl
RMSD bond length	0.002
RMSD bond angle	1.193
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0253)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.092
Number of reflections	75955	7451
<I/σ(I)>	21.4
Completeness [%]	99.9
Redundancy	7.4
CC(1/2)		0.751

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	5	313	Acetatic acid, Sodium formate